How Does The Male Gamete In Flowers Differ To The Animal Male Gamete?
Plant Indicate Behav. 2014; 9(12): e977715.
Gamete attachment process revealed in flowering institute fertilization
Toshiyuki Mori
aneWaseda Institute for Advanced Study; Waseda University; Tokyo, Nippon
#Present accost: Section of Biological Sciences; Graduate School of Science; The University of Tokyo; Tokyo, Japan
Tomoko Igawa
2Graduate School of Horticulture; Chiba University; Chiba, Nippon
Received 2014 Aug 11; Accustomed 2014 Sep four.
Abstract
Sex-possessing organisms perform sexual reproduction, in which gametes from different sexes fuse to produce offspring. In most eukaryotes, one or both sex gametes are motile, and gametes actively arroyo each other to fuse. Withal, in flowering plants, the gametes of both sexes lack motility. Two sperm cells (male gametes) that are independent in a pollen grain are recessively delivered via pollen tube elongation. Later on the pollen tube bursts, sperm cells are released toward the egg and central cells (female gametes) inside an ovule ( Fig. 1 ). The precise mechanism of sperm jail cell motion after the pollen tube bursts remains unknown. Ultimately, one sperm cell fuses with the egg cell and the other one fuses with the fundamental cell, producing an embryo and an endosperm, respectively. Fertilization in which two sets of gamete fusion events occur, called double fertilization, has been known for over 100 y. The fact that each morphologically identical sperm cell precisely recognizes its fusion partner strongly suggests that an accurate gamete interaction organization(south) exists in flowering plants.
Illustration of the fertilization process in flowering plants. First, each pollen tube accesses an ovule containing egg and central cells. Adjacent, the 2 sperm cells face up the female person gametes in the ovule after the pollen tube bursts. Finally, each sperm cell simultaneously fuses with either egg or fundamental cell.
Keywords: fertilization, gamete attachment, gamete fusion, GEX2, male person gamete, sexual reproduction, sperm cell
Fertilization Mechanisms Reside on the Gamete Surface of Flowering Plants
Gamete fusion is a mysterious phenomenon in which two gametes, derived from dissimilar jail cell lineages, successfully recognize, dock and merge with each other. This mechanism is largely conserved in plants and animals.1 We therefore speculate that each sex gamete is programmed to limited surface factors that specifically regulate fusion between unlike sexes. Indeed, previous studies on fertilization take identified several gamete surface factors critical for gamete recognition, zipper and fusion.i,ii Mori and colleagues identified a male-specific gamete fusion factor called GENERATIVE Jail cell SPECIFIC ane (GCS1), also known equally HAPLESS2 (HAP2), in lily pollen generative cells.iii,4 GCS1 is a novel type I transmembrane protein, possessing an Due north-terminal signal sequence and a transmembrane domain. It is exclusively expressed in male gametes.3 GCS1-knockout Arabidopsis thaliana sperm cells are sterile, unable to fuse with any of the female person gametes in an ovule. GCS1 therefore became the first example of a gamete surface factor that regulates double fertilization.3 At the aforementioned time, this finding predicts that additional factors surrounding GCS1 reside in both sex gametes. Molecular orchestration of these factors leads to successful double fertilization. To identify these factors, Mori and colleagues performed a new experiment using Arabidopsis gametic marker lines.5
Identification of Angiosperm Gamete Attachment Factor, GEX2
A transgenic Arabidopsis plant, expressing both central jail cell specific GFP and sperm nucleus specific RFP, was mutagenized by ethyl methanesulfonate (EMS) to screen for mutants with lacking gamete fusion.5 A mutant candidate with an obvious gamete fusion deficiency, designated Y47, was detected. After mapping, a point mutation was found in the GAMETE EXPRESSED 2 (GEX2) gene. Therefore, the Y47 line was renamed gex2–1. Although GEX2 was previously reported to be a sperm specific poly peptide localized to the cell membrane, its role was unknown.six The phenotype of gex2–i plants provided the first evidence that GEX2 is involved in gamete fusion.5 In addition, Mori and colleagues revised the previously described GEX2 cistron structure and ended that GEX2 is a type I transmembrane protein, like GCS1.five Detailed observation of gex2–1 sperm cells revealed that incomplete double fertilization (also known equally single fertilization), in which i sperm cell successfully fuses with an egg or a central cell, occasionally takes place. This finding suggests that GEX2 is involved in stabilizing gamete contact rather than performance in a critical gamete fusion step, such as membrane fusion.5 Indeed, gex2–1 sperm cells occasionally detached from female gametes, simply those of gcs1 rarely did ( Fig. 2 ).5 To further investigate gex2–i sperm behaviors, Mori and colleagues invented a uncomplicated method for gamete behavioral ascertainment, in which female gametophytic cells are separated by polysaccharide-digesting enzymatic treatment.5 As a outcome, gex2–one sperm cell disengagement from egg and central cells was occasionally detected. This method allowed for statistical evaluation of the frequency of gamete detachment. However, most gcs1 sperm cells remained attached to female gametes. From these data, it was ended that GEX2 protein is a gamete zipper factor expressed on the sperm cell surface, providing the beginning evidence of a gamete zipper process in flowering plants.5 Although the spatiotemporal relationships betwixt GEX2-dependent attachment and GCS1-dependent fusion processes remain elusive,7 successful fertilization in flowering plants undoubtedly requires gamete contact preceding (or supporting) the fusion process, every bit in other organisms.8,ix
Gamete attachment step, revealed by analyses of Arabidopsis fertilization mutants. GCS1- or GEX2-knockout sperm cells expressing sperm nucleus RFP (snRFP) were observed in ovules expressing egg membrane GFP (emGFP). The top panels draw 2 pairs of GCS1-knockout (gcs1) sperm cells released from 2 pollen tubes, most likely because the first pollen tube failed to fertilize and the 2d pollen tube, which was guided past the fertilization recovery system,16 similarly failed. All the unfused gcs1 sperm cells are attached to the egg jail cell. In contrast, GEX2-knockout (gex2–1) sperm cells occasionally detached from the egg cell (bottom panels, arrowhead). The scale bar represents 20 μm.
GEX2 is Vascular plant-Specific and Possesses a Filamin-Like Domain(s)
According to the revised GEX2 gene construction, GEX2 is a type I transmembrane protein composed of an N-last signal sequence, filamin-like domains and a C-terminal transmembrane domain.v The filamin domain was originally divers in filamin proteins that office in the crosslinking of actin filaments. They are known to grade an immunoglobulin-like fold in humans and Dictyostelium discoideum (slime mold).10 The filamin domains are repetitive and grade an arm construction in a filamin molecule.ten GEX2 proteins are nowadays in multiple flowering plants, and all possess at to the lowest degree ane filamin-like domain ( Fig. iii ).5 In addition to flowering plants, the lycophyte Selaginella moellendorffii also possesses putative GEX2 and GCS1 orthologs ( Fig. iii and not shown, respectively). The Selaginella GEX2 structure contains at to the lowest degree five copies of the filamin-like domain ( Fig. iii ). Although it is unknown whether Selaginella GEX2 and GCS1 role similarly in fertilization, the presence of GEX2 and GCS1 orthologs in the Selaginella genome suggests that gamete attachment based on GEX2 has been established since the emergence of archaic vascular plants and that angiosperm GEX2 has been inherited from that of motile sperm. Furthermore, the differences in copy number and master structure of detectable filamin-like domains among plant species may have triggered reproductive isolation and speciation.five
GEX2 structures of vascular plants. All the GEX2 proteins identified in vascular plants are type I transmembrane proteins composed of an N-terminal betoken sequence (SS), filamin-like domain(southward) (FLMN) and a C-terminal transmembrane domain (TM). Amino acid positions of those domains are indicated. Accession numbers are as follows: {"type":"entrez-nucleotide","attrs":{"text":"AB743888","term_id":"576047781","term_text":"AB743888"}}AB743888 (Arabidopsis), {"type":"entrez-protein","attrs":{"text":"BAD33437","term_id":"50725909","term_text":"BAD33437"}}BAD33437 (Oryza) and {"type":"entrez-protein","attrs":{"text":"XP_002994042","term_id":"302824805","term_text":"XP_002994042"}}XP_002994042 (Selaginella). SS and TM domains were predicted by SOSUI (http://harrier.nagahama-i-bio.ac.jp/sosui/) and FLMN domains were predicted by PROSITE (http://prosite.expasy.org/).
Gamete Attachment Mechanisms are Both Alternative and Conservative
The green alga Chlamydomonas reinhardtii possesses 2 mating types (i.e., sexes) designated mt+ and mt-. When mating, mt+ gametes extrude a tube-shaped protrusion (tubular mating structure; TMS) to dock with mt- gametes ( Fig. 4 ).11 A blazon I transmembrane protein, FUS1, has been identified as an mt+ specific gamete attachment cistron. It is expressed in TMSs.ix,12 Wild type TMSs are capable of adhering to the mating mt- gametes, but FUS1-knockout TMSs are not.ix In the mt- gametes, GCS1 is specifically expressed and regulates the post-zipper step leading to gamete membrane fusion.13 GCS1-knockout mt- gametes retain the ability to adhere to wild type mt+ gametes.13 These data suggest that FUS1 and GCS1 do not bind to each other. Recently, it was found that a mammalian sperm specific type I transmembrane protein, IZUMO1, binds JUNO, a glycophosphatidylinositol (GPI)-anchored protein expressed in eggs, for successful fertilization ( Fig. 4 ).14,15 Because neither IZUMO1-knockout sperm nor JUNO-knockout eggs are fertile, this provided the first case of a direct interaction between male and female person gamete fusion factors that is essential for fertilization.15 In addition, it is likely that the IZUMO1-JUNO interaction functions only in gamete zipper.15 Cultured somatic cells expressing JUNO were able to adhere to those expressing IZUMO1 only non able to fuse.fifteen GEX2, FUS1 and IZUMO1 are specific to vascular plants, green algae and mammals, respectively, and their chief structures are quite different, suggesting that they arose independently in eukaryotic development. Nonetheless, it is withal noteworthy that these proteins are all type I transmembrane proteins containing immunoglobulin-like domain(s) and nigh probable serve as gamete attachment factors ( Fig. 4 ). The nature of gamete attachment systems might unexpectedly exist conserved amongst phylogenetically distant eukaryotes.
Gamete attachment factors containing immunoglobulin (IG)-like domain(s). Filamin-like domains are known to form an IG-similar fold. Both GEX2 and FUS1 possess filamin-like domain(s). IZUMO1 is a member of the IG superfamily and possesses an IG-like domain.
Funding Statement
This study was supported by a Grant-in-Aid for Scientific Research on Innovative Areas to T.Thousand. (21112008) and a Grant-in-Aid for Young Scientists (B) to T.Thousand. (24770062).
Disclosure of Potential Conflicts of Involvement
No potential conflicts of interest were disclosed.
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